Glandular extractive product and process of manufacturing same.



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TJEEUhllAfi n. AJDWMCH, @h DETRGPTT, MICHIGAN, nssrun'on T0 FARM, DAVIS & UUlllUPANY, UFMETROIT, MICHIGAN, A CURJPORATI'UN Uh MICHIGAN.

WEAJWDULAH. EXTRAETIVE JE'JLtUJD'UC'Jl AND PROCESS 01F MANUFAUTURHATW SAME. V

To all whom it may concern."

Be it hown that l, Tnorms B. Anonron, a citizen of the United States, residing at Detroit, in the county of Wayne and State of Michigan, have invented certain new and useful lmprovements in Glandular Extractive Products and Processes of Manufactur ing Same, of which the following is a specification. Y

My invention relates to anew and useful product and the process of making the same, as pointed out in the claims concluding these specifications.

A water extract of the therapeutically active substance contained in the infundibular or posterior lobe of the pituitary gland, is at the present time made and sold by difl'erent manufacturers under difi'erenttradenames, such as pituitrin; pituglandol; pituitary extract vaporole, etc. This extract, when administered hypodermically, has the edect of increasing the blood pressure and of stimulating uterine contractions. As a uterine stimulant its efl'ect is especially pronounced just at the time of natural childbirth, and it is at present largely used for that purpose. In this respect it diders from ergot. The blood pressure efiect is similar to that produced by adrenalin (the bloodpressure-raising, hemostatic and astringent principle of suprarenal glands) but is much more prolonged.

The above extract has been found to be very unstable-that is, it decomposes on standing and becomes less and less edective.

It is, therefore, desirable to isolate the active ciple from the same by a suitable precipitent, such as alcohol (either. ethyl or methyl), acetone or a mixture thereof. As

specification or Letters rate-mt.

Patented duly 2, lldld.

Application filed March 3, 1913!. serial lilo. 75inch.

is then reduced to a pulp and dried at body temperature, if desired. It may then, if de sired, be dehydrated and defatted with acetone or some other suitable solvent. The dried material may then be finely powdered and about ten parts of glycerin, say, 50% glycerin (that is, one part gylcerin to one part water) added. The extract may be obtained by macerating, shahng or grinding, with or without heat to, say, 50 C. After the glycerin extract has been in con tact with the gland material for a number of days, it is subjected to hltration-the filtration going on fairly rapidly when 50% glycerin is employed. Theextraction may be repeated as often as required. When a clear solution is obtained, one may add to one part of glycerin extract, say, three parts of 95% to 98% alcohol, agitate, and then add, say, four parts of acetone. After the precipitate has subsided the supernatant liquid may be decanted and the precipitate washed with successive small portions of acetone. Place in a vacuum-desiccator to remove traces of acetone and eventually reduce to a powder if not already in that form. The resultant is a dudy, white or nearly white powder. That the product is a highly potent one therapeutically and is at least a close approximation to, if not the chemically pure active principle of the pituitary gland, maybe judged from the fact that a fraction of a milligram of the substance is an ethcient dose on the occasion of human childbirth.

A solution of the product is precipitated by most, if not all, of the so-called alka' loidal precipitants, such as phospho-tungstic acld, picric acid, mercuric cblorid, mercuric potassium iodid, platinic chlorid, etc. it

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lib

gives what is known as Paulys reaction,

similar to histidin. lit gives with Millons reagent the characteristic reaction for proteid in which tyrosin is present. It gives the biuret reaction. lit is soluble in water, preferably acidulated, and glycerin of all strengths; but is insoluble in strong alcohol, acetone, etc.

What l claim and desire to secure by LEA ters latent of the United States is l. A substance possessing pressor and oxytocic activity extracted from the infundibular lobe of the pituitary gland such as can be obtained by the herein described process which comprises making a glycerin extract of said lobe and precipitating the substance from said extract With alcohol or acetone.

2. The process of extracting a substance possessing pressor and oxytocic activity from the infundibular lobe of the pituitary gland Which consists in making a glycerin extract of said lobe and precipitating the said material therefrom by a suitable precipitant.

3. The process of extracting a substance possessing pressor and oxytocic activity from the infundi'bular lobe of the pituitary gland which consists in dehydrating said lobe making a glycerin extract thereof and prei srinii cipitating the said material by a suitable precipitant.

4. The proces of extracting a substance possessing pressor and cxytocic activity from the infundibular lobe of the pituitary gland which consists in dehydrating and defatting said lobe making a glycerin extract thereof and precipitating the said material therefrom by a suitable recipitant.

In testimony Whereo' I have hereunto signed my name in the presence of the subscribing Witnesses.

THOMAS B. ALDRICH.

Witnesses:

J. Enema BULL, M. E. MGNINCH. 

